The BioBrick Assembly Manual provides step-by-step instructions for assembling BioBrick parts using the BioBrick Assembly Kit. To read more about the BioBrick system and browse the BioBrick collection, visit the Registry of Standard Biological Parts.
Get the kit from New England Biolabs. Download the manual or browse it below.
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If you need to design new BioBrick parts, please check out Ginkgo's part design tool that will automatically generate primers for construction of BioBrick parts by PCR.
For additional technical support relating to BioBrick assembly, please contact Ginkgo BioWorks Customer Support.
For technical support relating to the enzymes and buffers in the BioBrick Assembly Kit, please contact NEB at
Knight, T.F. Idempotent Vector Design for Standard Assembly of BioBricks. MIT Synthetic Biology Working Group Technical Report (2003).
Shetty, R.P., Endy, D., Knight, T.F. Engineering BioBrick vectors from BioBrick parts. J Biol Eng 2:5 (2008).
Canton, B., Labno, A., Endy, D. Refinement and standardization of synthetic biological parts and devices. Nat Biotechnol 26:787-93 (2008).